The effect of regional policies on the effectiveness of rights protection of labor unions is the important content of labor relation research. However, policy environment constantly changes, and personal characteristics of the new-generation migrant workers like education level and social inclusion ability are different from ones of the first-generation migrant workers, so related research needs to be extended. By game theory and ordered probit model, this paper confirms that if the signals are not sufficient, the adoption of adequate regional incentive policy can only ease tight labor relations and does not necessarily achieve the purpose of improving social welfare; wages negotiations, business environment and social services have significant effects on the satisfaction of labor union participation by new-generation migrant workers. And the effects of individual feature variables such as education level, social inclusion ability and legal knowledge vary with regions. Therefore, the exertion of positive effect of regional policies on rights protection of labor unions of new-generation migrant workers has the weeds for adequate information and effective cooperation with other factors.